microbiome and metagenome sequencing
by admin
on November 27, 2024

Microbiome and Metagenome Sequencing Services

Explore the forefront of microbiome and metagenome sequencing with MR DNA, your trusted partner for comprehensive sequencing solutions. Our advanced technologies, including Illumina and PacBio sequencing platforms, empower researchers to delve into the intricate ecosystems of bacteria, fungi, archaea, and viruses. Whether studying the human gut microbiome, soil metagenomes, or marine ecosystems, our services provide high-resolution data to uncover microbial diversity and functional potential.

At MR DNA, we specialize in 16S rRNA, ITS, COI, and functional gene sequencing, enabling precise taxonomic and functional profiling of microbial communities. With long-read sequencing options for comprehensive metagenomic assemblies, we bridge the gap between species-level resolution and functional gene analysis. From sample processing to advanced bioinformatics, our team delivers actionable insights for clinical, agricultural, and environmental research.

Harness the power of microbiome and metagenome sequencing to transform your research. Trust MR DNA to provide unparalleled accuracy, customized analyses, and fast turnaround times. Contact us today to learn how we can help unlock the potential of your microbial data. Visit us at mrdnalab.com.

Comprehensive Microbiome and Metagenome Sequencing Solutions

Unlock the secrets of microbial ecosystems with MR DNA, the leader in microbiome and metagenome sequencing. Our cutting-edge services are tailored to researchers exploring the vast diversity and functionality of microbial communities in health, agriculture, and the environment. From 16S rRNA gene sequencing for bacterial and archaeal profiling to ITS sequencing for fungal diversity, our platforms provide unparalleled resolution to uncover microbial complexity.

For deeper insights, our metagenome sequencing services enable full genomic analysis of complex samples, offering detailed views into microbial structure, metabolic pathways, and interactions. Utilizing long-read technologies like PacBio HiFi and Illumina short reads, we deliver high-quality assemblies that reveal both known and novel genes. Whether you’re analyzing soil, water, clinical samples, or the human microbiome, our team provides the expertise and tools to accelerate discovery.

With custom primer options, advanced bioinformatics pipelines, and functional gene profiling, MR DNA ensures you achieve accurate, meaningful data tailored to your study. Trust us to transform raw sequencing data into actionable insights. Discover the power of precision microbiome and metagenome research today with MR DNA at mrdnalab.com. Contact us to take your research further.

Here is a list of commonly used primers and their sequences for microbiome research:

16S rRNA Primers

  1. 27F: AGAGTTTGATCMTGGCTCAG
  2. 1492R: TACGGYTACCTTGTTACGACTT
  3. 341F: CCTACGGGNGGCWGCAG
  4. 805R: GACTACHVGGGTATCTAATCC
  5. 515F: GTGCCAGCMGCCGCGGTAA
  6. 806R: GGACTACHVGGGTWTCTAAT
  7. 967F: CAACGCGAAGAACCTTACC
  8. 1193R: ACGTCATCCCCACCTTCC
  9. 784F: AGGATTAGATACCCTGGTA
  10. 1061R: CRRCACGAGCTGACGAC

ITS Primers (Fungal)

  1. ITS1F: CTTGGTCATTTAGAGGAAGTAA
  2. ITS2: GCTGCGTTCTTCATCGATGC
  3. ITS3: GCATCGATGAAGAACGCAGC
  4. ITS4: TCCTCCGCTTATTGATATGC
  5. ITS5: GGAAGTAAAAGTCGTAACAAGG
  6. ITS1F (fungal-specific): TCCGTAGGTGAACCTGCGG
  7. ITS2R: GCTGCGTTCTTCATCGATGC

Functional Gene Primers

  1. amoA (Ammonia Monooxygenase): GGGGTTTCTACTGGTGGT
  2. nirS (Nitrite Reductase): CCTAYTGGCCGCCRCART
  3. nosZ (Nitrous Oxide Reductase): CGYTGTTCMTCGACAGCCAG
  4. mcrA (Methanogenesis Marker): GGTGGTGTMGGATTCACACARTAYGCWACAGC
  5. pmoA (Particulate Methane Monooxygenase): GGNGACTGGGACTTCTGG

Archaeal Primers

  1. Arch519F: CAGCMGCCGCGGTAAHACCVGC
  2. Arch806R: GGACTACVSGGGTATCTAAT
  3. 344F: ACGGGGYGCAGCAGGCGCGA
  4. 915R: GTGCTCCCCCGCCAATTCCT

Custom Functional and Environmental Primers

  1. COI (CytB): GGWACWGGWTGAACWGTWTAYCCYCC
  2. 18S rRNA Universal (Eukaryotes): AACCTGGTTGATCCTGCCAGT
  3. dsrA (Dissimilatory Sulfite Reductase): ACSCACTGGAAGCACGGCGG
  4. hzo (Hydrazine Oxidoreductase): TAYTAYTGYGATGTGACWGC
  5. rpoB (RNA Polymerase Beta): AGCTTTCAGCTTGTTGTTGG
  6. cbbL (Rubisco Large Subunit): GATTCCGACTTACCACGGT
  7. 16S-23S ITS Region: CACACCGCCCGTCACA

Would you like a formatted file or specific applications for these primers?

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